In vitro regeneration of a mature leguminous liana (Bauhinia vahlii Wight & Arnott)
Dhar U; Upreti J
Plant Cell Reports
An in vitro propagation protocol has been developed from mature lianas of Bauhinia valii. Browning was the major obstacle in the establishment of cultures. Explants collected during the growing season (April‚ÄìJune) showed maximum browning; however browning was minimal during the dormant phase. This problem was circumvented by soaking the sterilized explants in a solution of antioxidant (50 mg l‚Äì1 ascorbic acid+75 mg l‚Äì1 citric acid). The explants were thereafter transferred to culture room conditions after an initial incubation in the dark at 4 ¬8C for 48 h. Shoot proliferation (58%) shoot number (4.5) and shoot length (35 mm) was best in Murashige and Skoog (MS) medium supplemented with 2.5 ŒºM kinetin + 100 mg l‚Äì1 adenine sulfate. Seasonal fluctuations significantly affected the proliferation potential of the explants. March‚Äì April was found to be the best season for shoot initiation. Microshoots were rooted on a half-strength growth regulator-free agar-gelled Murashige and Skoog medium after a dip in half-strength MS liquid medium containing 1-naph-thaleneacetic acid + indole-3-butyric acid (10 ŒºM). Rooted plantlets were potted and acclimatized under culture room conditions for 4 weeks before transfer to a polyhouse.