Passiflora edulis is a species among the third generation of emerging fruit lianas, and it has huge economic value and broad market prospects for China. However, owing to low awareness of the breeding of favorable varieties, coupled with its late domestication and subsequence artificial selection, there is a lack of favorable cultivars grown in China compared with other countries. The aim of this study was to establish an efficient regeneration method for maintaining the excellent characteristics of the F, hybrid between P. edulis f. edulis Sims and P. edulis f. flavicarpa Degener. In this study, the influence of explant type, medium composition, and plant growth regulators for in vitro propagation of P. edulis were studied with single factorial, complete combination, and orthogonal experimental designs. The results revealed an efficient regeneration protocol for P. edulis adventitious buds via two propagation methods, namely indirect callus -mediated bud organogenesis from leaf explants and direct basal stem cluster bud organogenesis from nodal segment explants. Although Olive medium (OM) supplemented with 1.5 mg L-1 6-BA, 0.3 mg L-1 IBA, and 1.5 mg L-1 KT, easily generated adventitious buds from leaf explants through dedifferentiation and redifferentiation, this propagation method had some problems such as the length of the procedure, the poor quality of adventitious buds obtained and its low efficiency of bud formation. However, on OM supplemented with 1.0 mg L-1 6 -BA and 0.1 mg L-1 IBA, direct bud organogenesis from nodal explants overcame these problems, with a regeneration coefficient of 7.0. In addition, etiolation of leaves occurred after three generations in proliferation culture. To solve this problem, the OM was modified by increasing ferric salt content by 0.5 times. Adventitious root induction was accomplished on OM by adding 0.3 mg L-1 IBA, yielding a 98% rooting rate. The survival rate was more than 95%. An effective and stable regeneration system was determined in the present study, providing a technical foundation for P. edulis propagation and maintaining crosses with favorable characteristics.